BIO 580 - Animal Cell Culture
(4 cr.) All aspects of the theory and practice of animal cell culture are covered in this advanced course. Lecture and laboratory cover basic aspects of cell culture in vitro, including mammalian, avian, fish and insect cells. Laboratory procedures include: preparation of complex and synthetic media; mass and single-cell culture; primary and established cell cultures; large-scale growth of cells; culture contaminants; cell preservation; growth factors; measurement of cell growth and viability; cell cloning; cell synchrony; cell-cycle analysis, karyotyping, mutant isolation; cell fusion/hybridomas; culture of specialized cells; virus propagation; production of specialized cell products; toxicity testing; cell transformation/immortalization; and DNA transfection. Prerequisite: BIO 605 or BIO 606; As Needed

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